• Safety in the laboratory • Documentation • Basic Statistics • Use and maintenance of equipment • Lab maintenance including cleaning, Waste management and restocking • Sterile Technique • Media preparation • Safe handling of oocyte and sperm • Sperm concentration and motility assessment • Diagnostic semen analysis with strict morphology • Testicular identification • Preparation of sperm for IVF and ICSI • In Vitro Fertilization • Assessment of oocyte maturational stage and viability • Oocyte Identification • oocyte Denudation • Hyaluronidase treatment • ICSI procedure whether utilizing ejaculate spermatozoa or testicular spermatozoa • Assessment of fertilization • Assessment of Cleavage • Blastocyst grading • Embryo Culture • In Vitro Maturation • Assessment of Oocytes after maturation • Slow cooling of embryos • Rapid Cooling of embryos (Vitrivication technique) • Thawing of slow cooled and rapid cooled embryos • Sperm cryopreservation and thawing • Embryos biopsy using laser • Assisted hatching • Culture of biopsied embryos

Faculty of Science, Cairo University